Schwein IgG anti-Human IgG (H+L)-Biotin, MinX Bo,Ca,Ck,Dg,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh
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Übersicht
Artikelnummer SwAHu/IgG(H+L)/Bio Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Andere, ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Hamster allgemein, Huhn, Hund, Kaninchen, Katze, Maus, Meerschweinchen, Pferd, Ratte, Rind, Schaf, Ziege
Format aus Antiserum, Azid-frei, IgG-Fraktion, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis dies schließt eine Reaktion in empfindlicheren Nachweistechniken nicht aus, fehlende Kreuzreaktivität (MinX) zu o.g. Spezies mittels radialer Doppelimmundiffusion bestimmt
Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user is choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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