Ziege Fab anti-Maus IgG2c (Fc)-DyLight 405, MinX keine
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Übersicht
Artikelnummer 115-477-188 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Durchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe)
Konjugation Maximale Anregung Maximale Emission Keine Kreuzreaktivität (MinX) mit Verdünnung Format 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
Anwendungshinweis empfohlenes Gewichtsverhältnis (w/w) Fab:Prim.Ak 1:1 (molares Verhältnis 3:1), für Komplexbildung mit Primärantikörper in Lösung geeignet, optimale Verdünnung des Komplex für Assay titrieren, Prim.Ak+Fab vortexen und 30 min bei RT inkubieren
Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2c but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
Conjugate
DyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use is limited to microscopes or flow cytometers equipped with a 405 nm laser and a 420 nm emission filter. Under these conditions, it is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity. The combination of DyLight 405, Alexa Fluor 488, Rhodamine Red-X, and Alexa Fluor 647 provides for maximum color separation. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor 488, Cy3, and Alexa Fluor 647. DyLight 405 conjugates are an excellent choice for blue-fluorescing secondary antibodies in multi-color labeling protocols.