Maus IgG anti-Human IgE (NI 307)-HRPO, MinX keine
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Übersicht
Artikelnummer MAHu/IgE/PO Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung Andere, ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Ascites, gereinigter Antikörper, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgE, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:250, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards.
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
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