Kaninchen IgG anti-Maus IgG+IgM+IgA (H+L)-TRITC, MinX Hu
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Übersicht
Artikelnummer RAM/mIg/TRITC Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (IHC)
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Antiserum, Azid-frei, IgG-Fraktion, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis dies schließt eine Reaktion in empfindlicheren Nachweistechniken nicht aus, fehlende Kreuzreaktivität (MinX) zu o.g. Spezies mittels radialer Doppelimmundiffusion bestimmt
Zweckbestimmung Produktlinie / Thema Ig-spezifische Ak / Sekundärantikörper, Nordic-MUbio / Veterinärmedizin
Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates, to demonstrate immunoglobulins or specific antibodies in cells and tissues, to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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