Highest Affinity & Sensivity: Biacore 3×10-10M
The mouse monoclonal antibody clone 13/45/31-2 (Isotype IgG1) detects N- and C-terminally His-tagged proteines as well as internal His-tags. With a high affinity of 3x 10-10M (BiacoreTM) it is optimally suited for a large variety of applications.
The antibody is one of the most cited His-Tag Antibodies world wide with more than 100 References.
The anti-His-epitope-tag clone 13/45/31/2 has been developed for optimal detection in cells and complex cell-lysates (Prof. Zentgraf, DKFZ, Heidelberg, Germany). Clone 13/45/31-2 shows a very high specificity and sensitivity against recombinant proteins with a tag of at least 6 histidines. Constructs with 4 additional histidines show 20- to 40-times increased affinity without any significant impact on the tertiary structur of the expressed protein.
Binding of the epitope-tag antibody is not influenced by the flanking aa-sequence of the constructs offering a broad choice in the selection of available expression vectors. The only requirement for antibody binding is the stereoscopic accessibility of the epitope
Ordering Information His-Epitop-Tag Antibody
| Ordering # | Quantity | Description |
|---|---|---|
| dia-900-100 | 100 µg | Unconjugated |
| dia-900-200 | 200 µg | Unconjugated (Value Pack 2x 100µg) |
| dia-910-1MG-ABF | 1 mg | Unconjugated Azide- and BSA-free |
| dia-900-FITC | 100 Tests | FITC-Conjugate |
| dia-900-BIOT | 100 µg | Biotin-Conjugate |
Application Chart His-Epitope-Tag Antibody
| Application | Antibody dilution | |
|---|---|---|
| Biacore | Biacore | n.A. |
| EM | Elektron mikroskopy | Assay dependent |
| FACS – Flow | Flow Cytometry | 1:500-1:2000 |
| IHC-F | Immunohistochemistry – Frozen | Assay dependent |
| IHC-P / FFPE | Immunohistochemistry – Paraffin | Assay dependent |
| IP | Immunoprecipitation | 4 µg/ml |
| WB (colorimetric) | Western Blot (colorimetric) | 1:100 – 1:10.000 |
| WB (ECL) | Western Blot (ECL) | 1:1.000 – 1:10.000 |