DIA-310 – Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue – 500 µl

Art.-Nr.
DIA-310

Specificity
CD31
Species Reactivity
Mouse
Swine
Host Species
Rat
Isotype
IgG2a
Clone
SZ31
Clonality (Mono-/Polyclonal)
monoclonal
Application
Immunohistochemistry (frozen sections)
Immunohistochemistry (Paraffin-embedded Sections)
Western Blot
Immunohistochemistry (IHC)
Conjugation
unconjugated
Format
lyophilisate
antibody purified (from culture supernatant)
Product line / Topic
CD markers
angiogenesis
immunohistochemistry
Intended Use
for Research Use Only
Temperature – Storage
2-8°C
Search Code
DIA310
Manufacturer / Brand
dianova

Reactivity:

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.

Background:

CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. Cluster of Differentiation 31 (CD31) is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 (Anti-CD31) eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.

Immunohistochemistry of mouse CD31 (PECAM-1) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of:

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

aorta

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

aortic origin

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

endocardium

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

small intestine

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of small intestine from mice (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Dünndarm aus der Maus (FFPE Gewebe).

small intestine

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse colon (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Dickdarm aus der Maus (FFPE Gewebe).

colon

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse brain (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Mäuse Gehirn (FFPE Gewebe).

brain

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse lymph nodes (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren von Lymphknoten der Maus (FFPE Gewebe).

lymph nodes

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse bone marrow (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren im Knochenmark der Maus (FFPE Gewebe).

bone marrow

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mesenteric vessels from mice (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren in mesenterialen Gefäße der Maus (FFPE Gewebe).

mesenterial vessels

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse kidney (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mäuse Niere (FFPE Gewebe).

kidney

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse pancreas (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Mäuse Pankreas (FFPE Gewebe).

pancreas

(pictures courtesy of Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin, Germany)

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse lung (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mäuse Lunge (FFPE Gewebe).

Lung

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse muscle (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren im Muskelgewebe der Maus (FFPE Gewebe).

Muscle

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse spinal (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren im Spinal der Maus (FFPE Gewebe).

Spinal

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse liver (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren in der Mäuse Leber (FFPE Gewebe).

Liver

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Adenocarcinoma

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Adenocarcinoma

(pictures courtesy of Prof. Dr. H. Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin, Germany)

Western Blot analysis

Immunoblot of extracts from murine lung, J558L cells and m-Lend cells using CD31 rat monoclonal antibody clone SZ31 (DIA-310 1:5.000) and goat anti-rat-HRP antibody (1:10.000).

Western blot detection (WB) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Lane:

  1. J558 cells (CD31-), 25μg lysate
  2. murine lung, 25μg lysate
  3. blank
  4. m-Lend cells (CD31+), 12.5μg lysate

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