Goat F(ab’)2 anti-Human IgG (Fc)-Alk. Phos., MinX Bo,Ms,Rb
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Overview
SKU 109-056-170 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:5,000 – 1:50,000, Western Blot (WB): 1:5,000 – 1:50,000
Format 0.05% NaN3, 10 mM Tris-HCl (pH 8,0), 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, mouse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
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