Goat IgG anti-Rabbit IgG (H+L)-HRPO, MinX Hu,Ms,Rt
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SKU 111-035-144 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Dilution Format Intended Use Product line / Topic Manufacturer / Brand
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Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.