Goat F(ab’)2 anti-Rabbit IgG (F(ab’)2)-Alexa Fluor 488, MinX Hu
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Overview
SKU 111-546-047 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Flow Cytometry 1:100 – 1:800, Histo-/Cytochemistry 1:100 – 1:800
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Intended Use Product line / Topic previous Catalog No. Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against the Fc portion of rabbit IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.