Goat IgG anti-Rat IgG (Fc)-Cy5, MinX Hu,Bo,Ho
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Overview
SKU 112-175-071 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins. No antibody was detected against rat IgM or non immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. However, we recommend Alexa Fluor 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 in aqueous mounting media. The reverse is true when mounting in non-polar, plastic mounting media, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor 647 and DyLight 650.