Goat IgG anti-Mouse IgG (Fc)-HRPO, MinX Hu,Bo,Rb
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SKU 115-035-164 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Dilution Format Application Note
does not react with IgG, F(ab')2 Fragment, does not react with immunoglobulin light chains, equal reactivity with subclasses 1+2a+2b+3, IgG, Fc gamma-specific, useful for the detection of 25kD proteins in Western blotting after immunoprecipitation
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with mouse IgG subclasses 1, 2a, 2b, and 3 but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.