Goat IgG anti-Mouse IgG (L)-Alexa Fluor 488, MinX Bo,Go,Ho,Hu,Rb,Rt,Sh
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Overview
SKU 115-545-174 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Flow Cytometry 1:100 – 1:800, Histo-/Cytochemistry 1:100 – 1:800
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note do not use for detection of lambda light chains, reacts predominantly with kappa light chains
Intended Use Product line / Topic previous Catalog No. Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.