Goat IgG anti-Mouse IgG2b (Fc)-Brilliant Violet 421, MinX Hu,Bo,Rb
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Overview
SKU 115-675-207 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Format 0.005% Tween 20, 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 150 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS pH7.2, lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on anitgen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
BD Brilliant Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor 488, Rhodamine-Red-X, and Alexa Fluor 647. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor 488 and Rhodamine Red-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5 (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5‘s excitation and emission profiles overlap those of Alexa Fluor 647.