Mouse F(ab’)2 anti-Rat IgG (H+L)-Alexa Fluor 594, MinX Hu,Bo,Ho,Ms,Rb,Go
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Overview
SKU 212-586-168 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Flow Cytometry 1:100 – 1:800, Histo-/Cytochemistry 1:100 – 1:800
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Intended Use Product line / Topic previous Catalog No. Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse, mouse, goat, and rabbit serum proteins. The antibody may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.