Mouse IgG anti-Sheep IgG (L) (20B07-02A09)-Alexa Fluor 594, MinX Ho,Hu,Ms,Rb,Rt
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Overview
SKU 213-582-177 Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Flow Cytometry 1:100 – 1:800, Histo-/Cytochemistry 1:100 – 1:800
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Intended Use Product line / Topic previous Catalog No. Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on sheep IgG and with those common to other sheep immunoglobulins. The antibody does not react with the heavy chain of sheep IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit, and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.