Rabbit IgG anti-Human IgM (µ) (RM121)-Biotin, MinX Hu
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Overview
SKU 31-1023-02 Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (IHC), Immunoprecipitation
Conjugation No Cross-reactivity (MinX) with Format Application Note Antibody concentration: 1mg/ml, No cross reactivity with human IgG, IgA, IgD, or IgE, Reacts to the heavy chain of human IgM
Intended Use Product line / Topic Temperature - Storage Manufacturer / Brand - Datasheets and Downloads
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Images
Sandwich ELISA using RM121 as the capture antibody(100ng/well), and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followedby an alkaline phosphatase conjugated streptavidin. Sandwich ELISA using RM121 as the capture antibody(100ng/well), and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followedby an alkaline phosphatase conjugated streptavidin. ELISA of human immunoglobulins shows RM121reacts only to human IgM. No cross reactivitywith Human IgG, IgE, IgD, or IgA. The plate wascoated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL of RM121 was used asthe primary antibody. An alka A titer ELISA using RM121. The plate was coatedwith different amounts of human IgM. A serial dilutionof RM121 was used as the primary antibody. An alkalinephosphatase conjugated anti-rabbit IgG as the secondaryantibody.
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