Rabbit IgG anti-Sheep IgG (F(ab’)2)-Rhod. Red-X, MinX Hu

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against the Fc portion of sheep IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

Conjugate

RRX (Rhodamine Red-X) conjugates have a peak of excitation at 570 nm and a peak of emission at 590 nm. Although TRITC has been used traditionally with FITC for double labeling, better color separation is achieved by using RRX or Alexa Fluor 594. Rhodamine Red-X is particularly useful for 3- and 4-color labeling with DyLight 405, Alexa Fluor 488, and Alexa Fluor 647 by using a confocal microscope equipped with a 405 nm laser and a krypton/argon laser. Fluorescence from RRX lies about midway between that of Alexa Fluor 488 and Alexa Fluor 647, and it shows little overlap with either dye. The krypton-argon laser emits lines at 488 nm, 568 nm, and 647 nm, which are optimal for exciting Alexa Fluor 488, RRX, and Alexa Fluor 647, respectively. By adding a 405 nm laser and a 420 nm emission filter, 4-color labeling is possible using DyLight 405-conjugated secondary antibodies from Jackson ImmunoReseach. The separation between all four dyes is perfect for 4-color labeling, and all four dyes are very bright.