Sheep IgG anti-Mouse IgG (Fc)-Biotin, MinX Hu,Bo,Ho
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Overview
SKU 515-065-071 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:20,000 – 1:400,000, Flow Cytometry 1:200 – 1:1,000, Immunofluorescence 1:200 – 1:1,000, Immunohistochemistry (IHC): 1:500 – 1:5,000, Western Blot (WB): 1:20,000 – 1:400,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note does not react with IgG, F(ab')2 Fragment, does not react with immunoglobulin light chains, IgG, Fc gamma-specific, useful for the detection of 25kD proteins in Western blotting after immunoprecipitation
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or non immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.