Donkey IgG anti-Goat IgG (H+L)-Gold 4nm, MinX Ck,Gp,Hs,Ho,Hu,Ms,Rb,Rt
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Overview
SKU 705-185-147 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Chicken, Guinea Pig, Hamster syrian, Horse, Human, Mouse, Rabbit, Rat
Dilution Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 150 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM BBS/PBS (pH 8.5), lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Since signal intensity is relatively independent of particle size when silver enhancement is used, Jackson ImmunoResaerch offers 4 nm particles because this size may penetrate tissues better than larger particles.
The 4 nm size may be used for electron microscopy in studies that require smaller particles since they are relatively uniform in size (coefficient of variation less than or equal to 15%), though small aggregates are not removed from this grade. Therefore, the 4 nm particles should only be used for electron microscopy with the understanding that the presence of small aggregates may give misleading results.
A detailed protocol for silver enhancement is provided with all orders for LM Grade products. All reagents involved in the light-insensitive silver enhancement reaction can be prepared easily in the laboratory. However, those who wish to use commercially available silver enhancement kits can continue to do so. All LM Grade colloidal gold-protein complexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution, they may be frozen in aliquots for extended storage