Anti-CD31 (Ms,Sw) from Rat (SZ31) – unconj. for mouse FFPE tissue
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Overview
SKU DIA-310 Specificity Species Reactivity Immunogen Murine amino acid fragment (amino acids 610-681 of mouse CD31)
Host Species Isotype Clone Clonality (Mono-/Polyclonal) Application Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation Dilution Immunohistochemistry (IHC): 1:20, Western Blot (WB): 1:5,000
Format 0.05% NaN3, 2% BSA, in PBS (pH 7.4), lyophilisate, purified antibody (from culture supernatant)
Application Note Product line / Topic Intended Use Temperature - Storage Temperature - Transport Search Code Manufacturer / Brand Uniprot_ID Gene_ID Alias CD31, EndoCAM, GPIIA', PECA1, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/Endothelial Cell Adhesion Molecule, Platelet/Endothelial Cell Adhesion Molecule 1
- Datasheets and Downloads
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Additional Product Information
Reactivity:
Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections. Clone SZ31 shows no cross-reactivity to human CD31.
Outstanding specificity of clone SZ31 has been confirmed in more than 185 citations (CiteAB).
Background:
CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. Cluster of Differentiation 31 (CD31) is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 (Anti-CD31) eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.
Immunohistochemistry of mouse CD31 (PECAM-1) in formalin-fixed paraffin-embedded tissue sections
The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of:
aorta
aortic origin
endocardium
small intestine
small intestine
colon
brain
lymph nodes
bone marrow
mesenterial vessels
kidney
pancreas
(pictures courtesy of Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin, Germany)
Lung
Muscle
Spinal
Liver
Adenocarcinoma
Adenocarcinoma
(pictures courtesy of Prof. Dr. H. Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin, Germany)
Western Blot analysis
Immunoblot of extracts from murine lung, J558L cells and m-Lend cells using CD31 rat monoclonal antibody clone SZ31 (DIA-310 1:5.000) and goat anti-rat-HRP antibody (1:10.000).
Lane:
- J558 cells (CD31-), 25μg lysate
- murine lung, 25μg lysate
- blank
- m-Lend cells (CD31+), 12.5μg lysate
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