Anti-p16 (CDKN2A) (Hu) from Mouse (JAP16) – unconj.
Estimated delivery time: 10 business days
SKU DIA-P16-OD Specificity Species Reactivity Host Species Isotype Clone Clonality (Mono-/Polyclonal) Application Conjugation Dilution Format Immunogen Product line / Topic Intended Use Temperature - Storage Temperature - Transport Search Code Manufacturer / Brand Alias
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dianova develops under the trademark “ONCOdianova” new antibodies for IHC detection of cancer biomarkers in FFPE-tissues.
Anti-p16 antibody clone JAP16 has been developed and validated for IHC detection of p16 (INK4A) tumor suppressor in routine-fixed FFPE tissue sections.
p16 is a strong negative regulator of cell proliferation and a critical tumor suppressor protein. It is the principal member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors. During cell cycle progression, p16 specifically inhibits the formation of an active CDK4/6 kinase complex and thus, phosphorylation of retinoblastoma (Rb) protein. Since phosphorylation of Rb protein is a vital step for G1-to-S phase transition, p16-binding to the upstream kinase leads to cell cycle arrest.
p16 inactivation has been reported in nearly 50% of all human cancers, e.g.: head and neck, esophagus, biliary tract, liver, lung, bladder, colon and breast carcinomas, leukemia, lymphomas, glioblastomas. Besides downregulation, p16 overexpression has been observed in HPV (human papilloma virus)-related tumors such as cervical cancer. HPV oncoproteins target the p16-Rb pathway by E7 induced degradation of the retinoblastoma protein. Here, the overexpression of p16 reflects an unsuccessful attempt to stop cell proliferation in HPV-related tumors.
p16 is an important immunohistochemical (IHC) marker in gynecologic pathology.
Immunohistochemistry of human p16 (INK4A) in routine formalin-fixed paraffin-embedded (FFPE) tissue samples
A: Intense nuclear p16 staining in mesenchymal components of a phyllodes tumor of breast
B: Cytoplasmic and nuclear p16 staining in a cervical adenocarcinoma
C: Diffuse positive signal for p16 in an endometrioid ovarian carcinoma
D: Intense p16 staining in a serous ovarian carcinoma
(A) Phyllodes tumor of breast
B) Cervical adenocarcinoma
(C) Endometrioid ovarian carcinoma
(D) Serous ovarian carcinoma
(pictures courtesy of Prof. Guido Sauter, Department of Pathology, University Hospital Eppendorf, Hamburg, Germany)
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