Donkey IgG anti-Goat IgG (H+L)-unconj., MinX Ck,Hm,Ho,Sw

The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against goat serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user is own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.