Goat IgG anti-Guinea Pig IgG2 (Fc)-Biotin, MinX none
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Overview
SKU GAGp/IgG2/Bio Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin-embedded Sections), others, Western Blot
Conjugation No Cross-reactivity (MinX) with Format Azide free, from Antiserum, IgG-fraction, in PBS (pH 7.2), lyophilisate
Application Note Intended Use Product line / Topic Nordic-MUbio / Secondary Antibodies, Nordic-MUbio / Veterinary Medicine
Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is directed to the subclass IgG2. It does not react with IgG1, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user is choice has to be usedThis immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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