Goat IgG anti-Human IgG+IgM+IgA (H+L)-Biotin, MinX none

The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of human origin in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in human serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user is choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.