Goat IgG anti-Mouse IgG (F(ab’)2)-unconj., MinX Bo,Ca,Ck,Dg,Hm,Ho,Hu,Mo,Rb,Sh,Sw
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Overview
SKU GAM/Fab/7S Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Bovine, Cat, Chicken, Dog, Hamster generally, Horse, Human, Monkey, Rabbit, Sheep, Swine
Format Azide free, from Antiserum, in PBS (pH 7.2), lyophilisate, purified IgG Fraction
Application Note lack of cross-reactivity (MinX) to indicated species determined by double radial immunodiffusion, this does not exclude some reaction in more sensitive techniques
Intended Use Product line / Topic Ig specific Ab / Secondary Antibody, Nordic-MUbio / Veterinary Medicine
Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose.When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
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