Goat IgG anti-Rabbit IgG (Fc)-FITC, MinX none
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Overview
SKU GAR/IgG(Fc)/FITC Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (IHC)
Conjugation No Cross-reactivity (MinX) with Format Azide free, from Antiserum, IgG-fraction, in PBS (pH 7.2), lyophilisate
Intended Use Product line / Topic Ig specific Ab / Secondary Antibody, Nordic-MUbio / Veterinary Medicine
Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is restricted to the Fc part of the IgG molecule which expresses strict (class) specificity. In immunoelectrophoresis and radial immunodiffusion using various antiserum concentrations against rabbit serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgG used as immunogens. It does not react with IgA, IgG/Fab fragments and IgM or any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgG isotype in the middle layer of the indirect test procedure.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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