Mouse IgG anti-Human Bence Jones kappa, surface and hidden determinants (NI 285,NI 250)-unconj., MinX none

The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting,immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Delipidated, heat inactivated, lyophilized, stable whole ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.