Mouse IgG anti-Human IgA2 (NI 512,A89-038)-HRPO, MinX none

The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgA2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards.

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.