Mouse IgG anti-Human IgE (NI 307)-unconj., MinX none

The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.