Mouse IgG anti-Human IgG (Fc) (NI 335 + NI 343)-HRPO, MinX none

The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500, in ELISA from 1:1000 upwards, in Western blotting from 1:2000 upwards.

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.