Mouse IgG anti-Human IgM (µ) (NI 179)-unconj., MinX none
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Overview
SKU MAHu/IgM/MAb Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Format Azide/BSA free, from ascites, in PBS (pH 7.2), lyophilisate, purified antibody
Application Note Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is restricted to an isotype specific determinant on the IgM molecule as tested in ELISA, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgM immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. These data should be interpreted as general recommendations only.
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. No foreign proteins added Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
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