Mouse IgG anti-Human IgM (µ) (NI 179)-TRITC, MinX none
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Overview
SKU MAHu/IgM/TRITC Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (IHC)
Conjugation No Cross-reactivity (MinX) with Format from ascites, in PBS (pH 7.2), lyophilisate, purified antibody
Application Note Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunofluorescence staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.
Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
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