Mouse IgG anti-Human Secretory Component, free and bound (NI 194-4,A89-039)-Biotin, MinX none
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Overview
SKU MAHu/SC/Bio Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin-embedded Sections), others, Western Blot
Conjugation No Cross-reactivity (MinX) with Format from ascites, in PBS (pH 7.2), lyophilisate, purified antibody
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is restricted to secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user is choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.
Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
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