Mouse IgG anti-Human Secretory Component, free and bound (NI 194-4,A89-039)-unconj., MinX none

The reactivity of the antiserum is restricted to determinants on the secretory component as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of free or bound secretory component in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.