Rabbit IgG anti-Mouse IgG (Fc)-Biotin, MinX Bo,Ca,Ck,Dg,Go,Ho,Hu,Mo,Sh,Sw
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Overview
SKU RAM/IgG(Fc)/Bio Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin-embedded Sections), others, Western Blot
Conjugation No Cross-reactivity (MinX) with Bovine, Cat, Chicken, Dog, Goat, Horse, Human, Monkey, Sheep, Swine
Format Azide free, from Antiserum, IgG-fraction, in PBS (pH 7.2), lyophilisate
Application Note does not react with IgG, F(ab')2 Fragment, does not react with immunoglobulin light chains, lack of cross-reactivity (MinX) to indicated species determined by double radial immunodiffusion, this does not exclude some reaction in more sensitive techniques, useful for the detection of 25kD proteins in Western blotting after immunoprecipitation
Intended Use Product line / Topic Ig specific Ab / Secondary Antibody, Nordic-MUbio / Veterinary Medicine
Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user is choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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