Rabbit IgG anti-Mouse IgG (Fc)-HRPO, MinX Bo,Ca,Ck,Dg,Go,Ho,Hu,Mo,Sh,Sw
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Overview
SKU RAM/IgG(Fc)/PO Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin-embedded Sections), others, Western Blot
Conjugation No Cross-reactivity (MinX) with Bovine, Cat, Chicken, Dog, Goat, Horse, Human, Monkey, Sheep, Swine
Format Azide free, from Antiserum, IgG-fraction, in PBS (pH 7.2), lyophilisate
Application Note does not react with IgG, F(ab')2 Fragment, does not react with immunoglobulin light chains, lack of cross-reactivity (MinX) to indicated species determined by double radial immunodiffusion, this does not exclude some reaction in more sensitive techniques, useful for the detection of 25kD proteins in Western blotting after immunoprecipitation
Intended Use Product line / Topic Ig specific Ab / Secondary Antibody, Nordic-MUbio / Veterinary Medicine
Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity as tested against mouse serum and a panel of purified homogenous immunoglobulins In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in mouse serum or other body fluids, in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgG in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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