Find answers to frequently asked questions about your application. If you can not find what you are looking for, please do not hesitate to contact our technical service.
FAQ Storage Instructions for Antibodies and Proteins
In many cases it is not necessary to keep the goods cooled during shipping. However, after you receive the goods it is vital to observe the storage recommendations stipulated in the data sheet.
FAQ Dilution of Secondary Antibodies
Suggested dilution factors for secondary antibodies based on IgG-Form, immunoassay and conjugate.
FAQ Information on Fluorophores and their Conjugates
Selecting the right fluorophore for your experiment depends on multiple aspects.
FAQ Multiple Labeling with MinX Antibodies
Selection of antibodies for simultaneous detection of more than one antigen depends on at least two important criteria.
Western Blot: Troubleshooting Guide
Analyzing a western blot result can cause problems. To help you with your next blot we put common problems and solutions together in a troubleshooting guide.
FAQ Hapten-anti-Hapten Signal Amplification (IHC / FISH)
An important requirement for indirect multiple labelling of different biomolecules in one single tissue are primary reagents which act simultaneously and without cross reaction as target for secondary immunoconjugates. Well known haptens are for example biotin, digoxigenin, and the fluorochrome fluorescein isothiocynate (FITC).
FAQ Immunohistochemical Staining of Mouse Antibodies on Mouse Tissue
One of the major problems is the inability of anti-mouse secondary antibodies to distinguish between endogenous murine immunoglobulins in the tissue and the mouse primary antibody.