Goat IgG anti-Alpaca IgG (VHH)-Biotin, MinX Bo
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Overview
SKU 128-065-232 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:20,000 – 1:400,000, Flow Cytometry 1:200 – 1:1,000, Immunofluorescence 1:200 – 1:1,000, Immunohistochemistry (IHC): 1:500 – 1:5,000, Western Blot (WB): 1:20,000 – 1:400,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3, and with the VHH domain of llama IgG, subclasses 2 and 3. It also reacts with whole molecule alpaca and llama IgG1. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.
These antibodies react primarily with the Fc region, and are not recommended for detection of VHH antibodies.
Conjugate
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.