Mouse IgG anti-Human IgG2 (Fc) (NI 25-1,HP 6207)-FITC, MinX none
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Overview
SKU MAHu/IgG2Fc/FITC Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (IHC)
Conjugation No Cross-reactivity (MinX) with Format from ascites, in PBS (pH 7.2), lyophilisate, purified antibody
Application Note Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards.
Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 1.5. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
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