IFKine™ conjugated secondary antibodies have improved brightness, photostability and less unspecific background staining. Antibodies are raised in donkey and preadsorbed against serum of other species, which makes them predestined for fluorescence staining, especially for fluorescence multiple labelling.
IFKine™ fluorescence secondary antibodies are highly specific and are not prone for unspecific hybridization with other samples. As a result, up to three different antigens can be detected at the same time with a common immunofluorecence microscope.
Feature & Benefits
- Easy to use. Component optimized liquid solution package, easy and convenient
- Suitable for fluorscence multiple labelling. Donkey host, compatible with most primary antibodies, easy to block
- Minimum nonspecific reaction. Featured serum absorbtion of other species
- High quality guarantee. High brightness, photostability and pH tolerance
IFKine™ fluorophore: Improved brightness and photostability
IFKine™ secondary antibodies: Donkey host, suitable for multiple labelling
IFKine™ optimization: Minimum nonspecific binding with samples
IFKine™ optimization: Minimum nonspecific binding with samples
Ordering Information
| Product Name | Cat. No. | Application | Size | Spectra |
| IFKine™ Green Donkey Anti-Mouse IgG | A24211 | IF, FC | 100 / 500 µl | |
| IFKine™ Green Donkey Anti-Rabbit IgG | A24221 | IF, FC | 100 / 500 µl | |
| IFKine™ Green Donkey Anti-Goat IgG | A24231 | IF, FC | 100 / 500 µl | |
| IFKine™ Orange Donkey Anti-Mouse IgG | A24311 | IF, FC | 100 / 500 µl | |
| IFKine™ Red Donkey Anti-Mouse IgG | A24411 | IF, FC | 100 / 500 µl | |
| IFKine™ Red Donkey Anti-Rabbit IgG | A24421 | IF, FC | 100 / 500 µl |
IFKine™ innovative fluorophore
IFkine™ fluorophores are a new generation of unique fluorescent dyes, which allow the aquisiton of brighter and more stable fluorescence results. In multiple staining experiments, they exert better effects compared to other commercially available dyes (see table).
| Product Name | Ex/Em (nm) | Superior alternativ to | Spectra |
| IFKine™ Green | 493/518 | FITC, Cy2, Alexa 488, Dylight 488 | |
| IFKine™ Orange | 555/570 | Cy3, Rhodamin, Alexa 555, Dylight 549 | |
| IFKine™ Red | 591/615 | Texas Red, Alexa 594, Dylight 594 |
Unique donkey host source
IFKine™ secondary antibodies raised in donkey are compatible with most primary antibodies and are therefore especially suitable for multi-staining experiments. In addition, serum from one single species (donkey) can be used to for blocking, avoiding complicated and time-consuming additional blocking steps. This eliminates any potential obstacles, usually caused by usage of different secondary antibodies species in on single staining experiment.
Minimum nonspecific binding with samples
IFKine™ secondary antibodies specifically recognize IgG from rabbit, mouse and goat, and are preadsorbed against many other species, such as human, rat or mouse. Experiments demonstrate that these secondary antibodies have almost no cross-reactions with other serum proteins, avoiding any nonspecific binding with samples.
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Multiple Labeling with MinX antibodies